Fig. 5: NE gene expression-based molecular subtyping and immune infiltration status. | Cell Discovery

Fig. 5: NE gene expression-based molecular subtyping and immune infiltration status.

From: Single-cell and spatial proteo-transcriptomic profiling reveals immune infiltration heterogeneity associated with neuroendocrine features in small cell lung cancer

Fig. 5

a The expression of 48 NE marker genes in all tumor nest ROIs. b The GSVA analysis of the NE marker gene expression of all tumor nest ROIs within each patient (NE scores up and down). c Kaplan–Meier survival curves of 44 LS-SCLC patients based on median cutoff point of NE scores. d Intra-tumor heterogeneity of previously defined SCLC molecular subtypes in different ROIs within the same LS-SCLC based on the expression of ASCL1 (SCLC-A), NEUROD1 (SCLC-N), and POU2F3 (SCLC-P). None of the tumors expressed YAP1 in this cohort. e Immuno-oncological and metabolism-related protein expression in tumor nest (green), tumor stroma (orange), and para-tumor (blue) ROIs. f The aggregated patient-level expression of molecular subtyping markers ASCL1, NEUROD1, POU2F3 (left) and the aggregated patient level of immuno-oncological protein markers in the tumor nest (top) and tumor stroma (bottom). g Kaplan–Meier survival curves of 44 LS-SCLC patients based on median cutoff point of CD45 protein levels. h Spearman correlation coefficients between the level of CD45 protein and expression of molecular subtyping markers.

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