Fig. 6: SNRNP200 enhances glycolysis and glutathione metabolism via RNA splicing.

a Volcano plot of the polar metabolites profiled in control and SNRNP200-knockdown MDA-MB-231 cells. Significantly differentially abundant metabolites are color-coded by individual category. b Volcano plot of the lipid profiles of control and SNRNP200-knockdown MDA-MB-231 cells. Metabolites with significant differences are color-coded according to their respective categories. Furthermore, the proportions of five distinct lipid categories in SNRNP200-knockdown MDA-MB-231 cells were assessed. The data were statistically analyzed using the chi-square test (***P < 0.001). c Images of the RIs of ALDOA, GAPDH, and GSS. d Western blot analysis of the expression levels of SNRNP200, ALDOA, GAPDH, and GSS in control and SNRNP200-knockdown MDA-MB-231 and BT-549 cells. e Comparison of the ECAR between control and SNRNP200-knockdown MDA-MB-231 cells. f Diagram summarizing metabolic genes involved in glycolysis, the TCA cycle, and glutamate metabolism. The diagram visually depicts normalized metabolite levels (boxes) and their respective upstream metabolic enzymes (circles) undergoing intron retention induced by SNRNP200 ablation. UDP uridine 5’-diphosphate, G-1-P glucose 1-phosphate, G-6-P glucose 6-phosphate, Gal-1-P galactose 1-phosphate, Ri-5-P ribulose 5-phosphate, R-5-P ribose 5-phosphate, PEP phosphoenolpyruvate, α-KG alpha-ketoglutarate, G-3-P glycerol 3-phosphate, 1,3-BPG glyceric acid 1,3-biphosphate, NADP nicotinamide adenine dinucleotide phosphate, FA fatty acids, GL glycerolipids, GP glycerophospholipids, SP sphingolipids, ST sterol lipids.