Fig. 2: Mice lacking Med23 in the OL lineage display CNS hypomyelination. | Cell Discovery

Fig. 2: Mice lacking Med23 in the OL lineage display CNS hypomyelination.

From: Mediator MED23 controls oligodendrogenesis and myelination by modulating Sp1/P300-directed gene programs

Fig. 2

a Strategy for the generation of the OL lineage-specific Med23 deletion in mice. b Tail suspension test for Ctrl (Med23fl/fl) and Med23cKO (Med23fl/fl;Olig1-Cre+/–) mice at P14. c Appearance of optic nerves in Ctrl and Med23cKO littermates at P14. Scale bar: 1 mm. d, e In situ hybridization for Mbp and Plp1 in the spinal cord (SC), corpus callosum (CC), and cerebellum (CB) from Ctrl and Med23cKO mice at P7. Scale bars: 100 μm. f, i, l, o Representative electron micrographs of optic nerves in Ctrl and Med23cKO mice at P17, P28, P60, and P350. Scale bars: 1 μm. g, j, m, p The percentage of myelinated axons in Ctrl and Med23cKO optic nerves at the indicated stages. The data are presented as the mean ± SEM; n = 3 animals/genotype. Two-tailed unpaired Student’s t-test, ***P < 0.001. h, k, n, q Myelin g-ratio scatterplot vs axon diameters of the optic nerves in Ctrl and Med23cKO at indicated stages. n = 3 animals/genotype. (≥ 70 myelinating axons were counted for each mouse). Two-tailed unpaired Student’s t-test, P < 0.0001.

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