Fig. 3: Examination of PML RBCC dimer interactions.
From: Cryo-EM structure of PML RBCC dimer reveals CC-mediated octopus-like nuclear body assembly mechanism
a Detailed interactions in the hydrophobic interface of the PML dimer. b Slabbed view of PML1−PML2 interaction interface. The surfaces, defined by hydrophilic and hydrophobic residues, were colored in blue and yellow, respectively. c Fluorescence microscopy images of HeLaPml−/− cells expressing EGFP-PML, EGFP-PMLV123R, EGFP-PMLF177R, EGFP-PMLL178R, EGFP-PMLV123R,F177R and EGFP-PMLV123R,L178R. Scale bar, 5 µm. Statistical analysis of PML NB formation was conducted. All experiments were performed in independent replicates, with NB counts calculated from ≥ 15 nuclei. Data are means ± SEM. ****P < 0.0001 (derived from ordinary one-way ANOVA test). All experiments/results displayed in the main figure were conducted with PML isoform IV.
