Fig. 4: PML CC-driven polymerization.
From: Cryo-EM structure of PML RBCC dimer reveals CC-mediated octopus-like nuclear body assembly mechanism
a Illustration of the CC-based PML polymerization. The dimeric interaction pattern of the CC domain was predicted by AlphaFold2, and the PMLR/B1/B2 dimer represents the experimentally obtained cryo-EM structure. b SDS-PAGE analysis of the PML46−362 protein. c XL-MS analysis of the purified PML46−362 protein, with the identified inter-subunit XLs shown as red lines. The best E-value (1.00 × 10−2) and spec count of at least 2 were used as the threshold to remove extra XL-MS data with lower confidence. d Enlarged view of the anti-parallel PML146−360−PML246−360 interaction, with the detected XLs mapped on the model.
