Fig. 6: MAPK13-dependent PHGDH pS371 reduces SSP flux and GSH production. | Cell Discovery

Fig. 6: MAPK13-dependent PHGDH pS371 reduces SSP flux and GSH production.

From: MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury

Fig. 6

a‒c SK-Hep1 cells stably expressing shPHGDH were infected with lentivirus expressing Flag-PHGDH WT or Flag-PHGDH S371A. Cells then were treated with 0.5 mM H2O2 for 12 h. The serine and glycine contents (a), GSH and ROS level (b, c) in these cells were measured. d‒f SK-Hep1 cells stably expressing shPHGDH were infected with lentivirus expressing shNT or shMAPK13 and then infected with lentivirus expressing Flag-rPHGDH WT or Flag-rPHGDH S371D respectively. Cells then were treated with 0.5 mM H2O2 for 12 h. The serine and glycine contents in the cells (d), GSH and ROS level (e, f) in these cells were measured. Data represent means ± SD of three independent experiments. P values were determined by two-tailed Student’s t-test.

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