Fig. 7: Serine supplementation alleviates oxidative stress-associated acute or chronic liver injury. | Cell Discovery

Fig. 7: Serine supplementation alleviates oxidative stress-associated acute or chronic liver injury.

From: MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury

Fig. 7

a–c c57BL/6J mice were intraperitoneally injected with normal saline or 300 mg/kg APAP for 24 h. And at 0 h and 12 h, mice were treated with 200 mg/kg serine solution or normal saline (control) by intragastric administration (i.g.). Serum ALT and AST levels were detected by kit (a). Liver necrosis area in H&E-stained sections were circle and quantified (b). Positive liver cells in TUNEL-stained sections were quantified (c). Representative images of H&E staining or TUNEL staining were shown on the left panel and statistical analysis were shown on the right panel (n = 5 in control group and n = 6 in serine supplementation group). d‒f c57BL/6 J mice were fed with 0.1% DDC irradiated diet with or without 10% serine for 2 weeks. Serum ALT and AST levels were detected (d). Sirius Red staining (e) and TUNEL staining (f) were performed in liver sections from these mice (n = 6 per group). Data are means ± SD. Each point represents an individual mouse. P values were determined by two-tailed Student’s t-test.

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