Fig. 1: Screening and evaluation of Nbs directed against six MPXV membrane proteins.
From: Identification of neutralizing nanobodies protecting against poxvirus infection

a The MPXV particle contains approximately 20 membrane proteins that are distributed across the intracellular mature virus (IMV) or extracellular enveloped virus (EEV), with their respective functions indicated. b Six of these membrane proteins, which serve as targets for neutralization or are components in vaccine formulations, were selected as antigens for Nb screening. A comprehensive structural comparison was conducted on the extracellular domains of these proteins from MPXV and VACV. The different amino acid sites in VACV are labeled. c SDS-PAGE and Coomassie Brilliant Blue staining of the purified viral membrane proteins. d Forty-one Nbs were obtained and their neutralizing capabilities were assessed against the IMV or the EEV of VACV-WR. Each symbol represents the mean ± standard deviation (SD) of the triplicate of neutralizing efficacy. e The selected Nbs were fused with mouse IgG-Fc and their neutralizing potency was reassessed at a concentration of 50 μg/mL against IMV of VACV-WR. The bars represent the mean ± SD of the neutralization results from triplicate experiments.