Fig. 2: Generation and validation of multivalent and Fc-fused M1R-01.
From: Identification of neutralizing nanobodies protecting against poxvirus infection
a Structural representation of the monovalent (M1R-01), bivalent (Bi-M1R-01), trivalent (Tri-M1R-01), and Fc-fused (mFc-M1R-01) forms of M1R-01. b Purified Nbs were verified by SDS-PAGE and Coomassie Brilliant Blue staining. c–f Affinities of M1R-01 (c), Bi-M1R-01 (d), Tri-M1R-01 (e), and Fc-M1R-01 (f) were assessed using SPR. g, h Neutralizing activities of these four variants of M1R-01 against IMV of modified vaccinia Ankara (MVA) (g) or VACV-WR (h) were evaluated. i The neutralizing activity of Tri-M1R-01 and Fc-M1R-01 against MPXV. Data were expressed as the mean ± SD and are derived from one of three independent experiments. j Summary of the IC50 values obtained from the neutralization assays, with data representing the mean of three separate experiments.
