Fig. 5: Crystallographic structure of the Nb M1R-01 complexed with M1R.
From: Identification of neutralizing nanobodies protecting against poxvirus infection
a Cartoon representation of the crystal structure showing the Nb M1R-01 in complex with its target protein, M1R. The ectodomain of M1R is depicted based on the predicted full-length structure by AlphaFold2. M1R is depicted in three distinct colors: sky blue for α-helices, dark gray for loops, and forest green for β-strands. The Nb is shown in dark salmon. The transmembrane domain of M1R is demarcated by black dashed lines. b Close-up view of the surface of M1R interacting with M1R-01. The epitope of M1R-01 on M1R is footprinted with a black line. The corresponding CDRs are shown with the interacting residues highlighted with colored circles. c Sequence and secondary structure of M1R. The locations of helices and β-strands based on the M1R structure are shown, with the circles indicating the contacting residues on M1R. d The binding interface between the M1R and M1R-01. The hydrogen bonds are shown as black dashed lines.
