Fig. 6: Characterization of the binding epitopes for M1R-01 and 7D11.
From: Identification of neutralizing nanobodies protecting against poxvirus infection
a Classification of antibodies targeting M1R/L1R. b SPR-based epitope competition assay to assess the binding interference between M1R-01 and 7D11. One antibody was used to pre-block the immobilized antigen and allowed to reach saturation prior to the flow of the other antibody across the sensor surface. Individual binding events are color-coded. c, d The affinity of wild-type M1R (left) and a D35N mutant M1R (right) for mouse monoclonal antibody 7D11 (c) and Nb M1R-01 (d) was determined using SPR.
