Fig. 2: Viperin promotes SGK1 expression in myocardial cells.
From: Targeting Viperin prevents coxsackievirus B3-induced acute heart failure
a Western blot analysis of SGK1, Viperin and cytomembrane KCNQ1 levels in the heart tissues of the Ctrl (n = 3) and AHF (n = 3) mice from the experiment of Fig. 1g. b Viperin+/+ (n = 3) and Viperin−/− (n = 3) mice were infected with CVB3 (2 × 105 PFU per gram body, i.p.) for 7 days. Western blot analysis was used to analyze SGK1 and cytomembrane KCNQ1 levels in mouse heart tissues. c Western blot analysis of SGK1 levels in HL-1 cells transfected with control shRNAs (Ctrl) or shRNAs against Viperin (shViperin, #1, #2, and #3) and then infected with CVB3 (MOI = 10) for 12 h. d, e Western blot analysis of SGK1 protein (d) or RT-qPCR analysis of Sgk1 mRNA (e) in HL-1 cells transfected with increasing amounts of Flag-Viperin. f Western blot analysis of SGK1 levels in Viperin+/+ or Viperin−/− HL-1 cells infected with CVB3 (MOI = 10) for 12 h. g The heart tissues that have relatively high levels of Viperin from experiment in Fig. 1I were selected. Western blot analysis was used to analyze SGK1 and cytomembrane KCNQ1 levels in mouse hearts (n = 5). **P < 0.01 and ***P < 0.001 (two-tailed unpaired Student’s t-test). Data are shown as means ± SD of four biological replicates (e), or are representative of three independent experiments (a‒d, f, g).
