Fig. 3: Accumulation of p21high macrophages in senescent mice contributes to reduced rejection following kidney transplantation.
a Interaction net count plot of allograft cells revealed by CellChat, with the thickness of the lines representing the number of interactions between the two cell types. b Senescence marker genes (Cdkn1a and Cdkn2a) expression projected on t-SNE plots (cell type refer to Fig. 2a), with red denoting high expression and gray denoting low expression. c Proportion of p21high cells in all cell types. d Graft-infiltrated macrophage in Young WT, Old WT and Old G3 Terc−/− mouse allografts treated with PBS Liposome or Clodronate Liposome. e Representative image of PAS of Young WT, Old WT and Old G3 Terc−/− mouse allografts treated with PBS Liposome or Clodronate Liposome. Scale bar, 50 μm. f Quantitative analysis of healthy tubule count per field, n = 3/group. g Quantitative analysis of i, t and v scores according to Banff 2019, n = 3/group. h Representative image of CD8+ T cells (CD8, green) immunofluorescent staining of allografts. Scale bar, 50 μm. Quantitative analysis of CD8+ T cell numbers per field is shown on the right side, n = 3/group. i Flow cytometric gating of lymphocytes, living cells, single cells, and CD8+ T cells. Representative flow panels of IFN-γ+CD8+ T cells from allografts are shown. Quantitative analysis of the IFN-γ+CD8+ T ratios are shown on the right side, n = 3/group. j Proportion of p21high macrophage in all macrophages of Young WT mouse allografts treated with PBS Liposome or Clodronate Liposome, n = 3/group. Data are presented as mean ± SD. Statistical analysis was performed using two-tailed Student’s t-test (c–h). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS, not significant.
