Fig. 4: The knockout of SLN’s enhancer can reduce the cortical neuron maturation. | Cell Discovery

Fig. 4: The knockout of SLN’s enhancer can reduce the cortical neuron maturation.

From: Spatiotemporal 3D chromatin organization across multiple brain regions during human fetal development

Fig. 4

a The dynamic soft clustering of genes during the development of the human PFC region. b The GO biological process enrichment results for dynamic gene clusters of the human PFC region. c The gene expression heatmap for top differential genes between GW11 PFC and GW26 PFC. d The 10-kb resolution O/E heatmap and interaction tracks for SLN gene promoter along with H1 and PFC CTCF ChIP-seq and published CP/GZ ATAC peaks (GSE95023), and brain ATAC OCR regions (GSE149268). e The schematic cartoon for SLN enhancer knockout. Predicted cleavage sites for two guide RNA pairs are shown. f The gene expression level for control (n = 3) and SLN enhancer knockout samples (n = 3). Error bars represent mean ± SEM. ***P < 0.001 (two-sided t-test). g Immunostaining for PAX6/TUBB3/DAPI at the human cortical organoid development day 36. Scale bars, 50 μm. Representative images are shown from n = 3 independent replicates. h Barplots for the percentage of PAX6+ neuron progenitor cells and the percentage for TUBB3+ mature neuron cells in control (n = 3) and SLN enhancer-knockout samples (n = 3). Error bars represent mean ± SEM. n.s., no significance (two-sided t-test).

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