Fig. 1: The identification of lncRNA-Gm26793 distribution in the gastrula and its functional significance during mESCs differentiation.

a DELs across ectoderm, mesoderm, endoderm, and primitive streak regions in the E7.5 embryo. The average expression pattern of each gene group is presented on the right panel as corn plots. End endoderm, Mes mesoderm, Ect ectoderm, PS primitive streak, A anterior ectoderm, P posterior ectoderm, L1 anterior left lateral ectoderm, R1 anterior right lateral ectoderm, L2 posterior left lateral ectoderm, R2 posterior right lateral ectoderm, EA anterior endoderm, EP posterior endoderm, MA anterior mesoderm, MP posterior mesoderm. b The spatial distribution of Gm26793 transcript in the mouse gastrula. Experimental validation of Gm26793 expression through whole-mount in situ hybridization was also shown. c Schematic diagram showing the strategy for the genomic knockout of Gm26793. d The workflow depicts the stem cell differentiation program and sampling timepoints for WT and Gm26793 knockout cells. e qPCR (n = 3) analyses determining the relative expression changes of the Gm26793 transcript during spontaneous differentiation. f Time-course expression profiles of mesoderm-related genes during WT and GKO EB differentiation measured by qPCR. g PCA analyses of EB differentiated samples show distinct clustering patterns and differentiation trajectories of WT and GKO mESCs. The major differentiation trajectories of WT and GKO mESCs are highlighted in the arrow lines. h Heatmap showing stage-specific co-expression gene modules and their correlation to differentiation days. Each row corresponds to a co-expression module. The correlation coefficients and P values are shown in each square. Representative genes and top GO terms of the four combined module categories are listed on the right panel. Data are shown as means ± SEM. Two-way ANOVA analysis with Tukey’s test is used in e and f; **P < 0.01, ***P < 0.001; n represents biologically independent replicates.