Fig. 2: Gm26793 knockout in mESCs boosts the responsiveness to PrE differentiation signals. | Cell Discovery

Fig. 2: Gm26793 knockout in mESCs boosts the responsiveness to PrE differentiation signals.

From: Genomic locus of lncRNA-Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo

Fig. 2

a, b Immunofluorescence analyses showing the specific distribution of GATA6/GATA4-positive cells in the outer layer of GKO EBs collected at day 8. Quantification of fluorescent-positive cells is shown in b (n = 5). c Illustration showing the sample collection of outer and inner layers in WT and GKO EBs by Geo-seq for transcriptomic analysis. d DEG analyses identify the specific gene expression pattern for each dissected WT and GKO sample. Representative genes and GO terms of each DEG group are listed on the right panel. e The phylogenetic tree displaying the clustering of EB differentiation samples with mouse pre-implantation cell lineages as well as definitive endoderm and mesoderm lineages. The mouse pre-implantation, definitive endoderm and mesoderm transcriptomic data are collected from published datasets63,70. f The schematic diagram describing the differentiation protocol for XEN-directed differentiation from embryonic stem cells. g The morphologies of differentiated cells in bright field for both WT and GKO XEN cells at day 7. h Temporal expression dynamics of Gata6 and Gata4 during WT and GKO XEN differentiation. i, j Representative images showing the presence of GATA6/GATA4-positive cells in WT and GKO groups at day 3 and 7 during XEN differentiation. Quantification of the data is shown in j. All data are shown as means ± SEM. Student’s t-test analysis is used in b and j; Two-way ANOVA analysis with Tukey’s test is used in h; *P < 0.05, ***P < 0.001; n represents distinct EBs.

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