Fig. 4: Gm26793-mediated regulation is independent of the transcript and local transcriptional activity. | Cell Discovery

Fig. 4: Gm26793-mediated regulation is independent of the transcript and local transcriptional activity.

From: Genomic locus of lncRNA-Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo

Fig. 4

a The schematic diagram of the genomic information around Gm26793 locus and the related promoter knockout strategy. b Time-series expression of Gm26793 in GPKO and GKO + Gm26793 cells (Gm26793 overexpression in GKO cells) during XEN differentiation. c qPCR analyses of primitive endoderm-related genes in the indicated cell groups on XEN-D7. d Quantification of Gm26793 transcripts by RNA-seq analysis. e Transcriptomic correlation of XEN-D7 differentiation samples upon Gm26793 transcript downregulation or overexpression. f The workflow for knocking down and overexpressing Fgf15 during differentiation by lentivirus infection. g Bar plots showing the expression dynamics of Fgf15 in the indicated cell groups during XEN differentiation. WT + Fgf15_KD represents knockdown of Fgf15 in WT mESCs. GKO + Fgf15 represents conditional overexpression of Fgf15 in GKO mESCs. h qPCR analyses of primitive endoderm markers among respective cell types on XEN-D7. i Quantification of Fgf15 transcripts by RNA-seq analysis. j PCA analyses of XEN-D7 differentiation samples showing the transcriptome similarities of differentiated cells following the treatment with decrease and increase in Gm26793 and Fgf15 expression. k, l Heatmap showing the DEGs between the indicated cell groups. The expression changes of representative up- and down-regulated genes are listed on the right panel. Top GO terms of up- and down-regulated genes are shown in l. All qPCR data are shown as means ± SEM. Two-way ANOVA analysis with Tukey’s test is used in b, g; One-way ANOVA analysis with Tukey’s test is used in c, h; *P < 0.05, **P < 0.01, ***P < 0.001.

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