Fig. 6: Gm26793 knockout leads to pervasive epigenomic remodeling, which can be rescued by knocking down Cubn expression.

a Schematic diagram of epigenomic profiling among related mESCs. b Genome browser snapshot showing the increased chromatin accessibility, H3K27ac and H3K4me3 enrichment around the Cubn locus in GKO mESCs. c Volcano plot determining the differential distribution of chromatin accessibility (left), H3K27ac (middle), and H3K4me3 (right) between WT and GKO cells. Enhanced signals in GKO cells are highlighted in red, and decreased signals in GKO cells are highlighted in blue. d Venn diagram showing genomic regions with increased H3K27ac in GKO cells, which are analyzed and divided into H3K27ac^up/H3K4me3^pos and H3K27ac^up/H3K4me3^neg elements based on the existence of H3K4me3 modification. Genomic annotations of divided regions are presented as pie chart. e Heatmaps showing the signal enrichment of H3K27ac and H3K4me3 around selected genomic regions (H3K27ac^up/H3K4me3^pos: left; H3K27ac^up/H3K4me3^neg: right). Representative annotated genes for the selected regions are also shown. Identical genes are labeled in red. f GO term enrichment for the genes in H3K27ac^up/H3K4me3^pos group (left) and H3K27ac^up/H3K4me3^neg group (right). g Representative genome browser snapshot showing efficient recovery of epigenetic distribution in GKO + Cubn-KD cells. H3K27ac^up/H3K4me3^pos regions are highlighted in light red rectangles, H3K27ac^up/H3K4me3^neg regions are highlighted in light blue rectangles.