Fig. 6: Whole genome screening detecting the potential related pathways involved in regulating APC in C1QC⁺ RTMs.

a Schematic representation of the workflow for the genome-wide CRISPR/Cas9 screen. b Cumulative distribution function (CDF) of biological replicates of 5 representative samples of M0, MHC^low, and MHC^high cells. CPM, counts per million. n = 5 in each group. c Top hit genes of MHC^low and MHC^high cells. The P value was corrected by Benjamini-Hochberg test. d Gene ontology (GO) term analysis of MHC^low and MHC^high cells. e, f KEGG gene interaction network of the hit genes in MHC^low and MHC^high cells. Subnetworks (Neighborhoods) are colored and annotated with enriched functional categories. Gray lines, connections within a neighborhood; red lines, connections between neighborhoods. g, h Representative flow cytometric plot and quantification of MHC-II expression levels in ESRRA-KO THP-1 cells during macrophage polarization. Vector control and THP-1-Cas9 were used as control for comparison. Vector control indicates THP-1 cells infected with lentivirus carrying an empty plasmid lacking gRNA. gMFI, geometric mean fluorescence intensity. P values in h were determined using one-way ANOVA.