Fig. 2: Functional characterization of C3 oxidase and reductase. | Cell Discovery

Fig. 2: Functional characterization of C3 oxidase and reductase.

From: Decoding and reprogramming of the biosynthetic networks of mushroom-derived bioactive type II ganoderic acids in yeast

Fig. 2

a HPLC analysis of fermentation extracts of strains SC62-CK-r-CYP512W2-r (CK-r-CYP512W2-r), SC62-AfuSDR-r-CYP512W2-r (Afu-r-W2-r), and SC62-CsSDR-r-CYP512W2-r (Cs-r-W2-r). b HPLC analysis of fermentation extracts of engineered S. cerevisiae strains BJ5464-CK-r (BJ-CK-r), BJ5464-AKR1C2-r (BJ-AKR1C2-r), BJ5464-AKR1C4-r (BJ-AKR1C4-r), and BJ5464-FusC1-r (BJ-FusC1-r) after incubation with GA-TR for 48 h. c HPLC analysis of the fermentation extracts of strains SC62-CsSDR-AKR1C4-r (Cs-C4-r), SC62-CK-r (CK-r), SC62-CsSDR-AKR1C4-CYP512A2-r (Cs-C4-A2-r), and SC62-CYP512A2-r (A2-r). d Production of GA-Jb, GA-TR, and GA-Ja by strains Afu-C2, Afu-C4, Afu-Fu, Cs-C2, Cs-C4, and Cs-Fu after 120 h fermentation. All data represent the mean of three independent samples, and the error bars indicate the standard deviation.

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