Fig. 4: Functional characterization of GlAT. | Cell Discovery

Fig. 4: Functional characterization of GlAT.

From: Decoding and reprogramming of the biosynthetic networks of mushroom-derived bioactive type II ganoderic acids in yeast

Fig. 4

a HPLC analysis of the fermentation extracts of strains SC62-GlAT-r-CYP512W2-r (GlAT-r-W2-r) and SC62-CK-r-CYP512W2-r (CK-r-W2-r). b HPLC analysis of the fermentation extracts of strains SC62-GlAT-r-CsSDR-AKR1C4-CYP512W2-r (GlAT-r-Cs-C4-W2-r) and SC62-CK-r-CsSDR-AKR1C4-CYP512W2-r (CK-r-Cs-C4-W2-r). c HPLC analysis of the in vitro enzymatic extracts by incubating the GlAT-containing microsomes (prepared from the strain YL-T3-GlAT-r) with different substrates. CK indicates the microsomes prepared from the control strain YL-T3-CK-r.

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