Fig. 6: Functional characterization of BsAT. | Cell Discovery

Fig. 6: Functional characterization of BsAT.

From: Decoding and reprogramming of the biosynthetic networks of mushroom-derived bioactive type II ganoderic acids in yeast

Fig. 6

a HPLC analysis of the fermentation extracts of strains SC62-BsAT-r-CYP512W2-r (BsAT-r-W2-r), SC62-CK-r-CYP52W2-r (CK-r-W2-r). b HPLC analysis of the fermentation extracts of strains SC62-BsAT-r-CsSDR-AKR1C4-CYP512W2-r (BsAT-r-Cs-C4-W2-r) and SC62-CK-r-CsSDR-AKR1C4-CYP512W2-r (CK-r-Cs-C4-W2-r). c LC-MS analysis of the in vitro enzymatic reaction extracts by incubating BsAT-containing crude enzyme (prepared from the strain BL21-BsAT-r) with different substrates. CK indicates the crude enzyme prepared from the control strain BL21-CK-r.

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