Fig. 8: Reprogramming of the biosynthetic network to target TIIGAs. | Cell Discovery

Fig. 8: Reprogramming of the biosynthetic network to target TIIGAs.

From: Decoding and reprogramming of the biosynthetic networks of mushroom-derived bioactive type II ganoderic acids in yeast

Fig. 8

a Biosynthesis of target TIIGAs can be achieved by tunable expression of key enzymes either via changing the concentration of antibiotics (green), integration of key enzyme expression cassettes without antibiotics (blue), or inducible expression of acetyltransferases (yellow). For speculating the chemical structures corresponding to peaks 20, 21, 28–33, see Supplementary Figs. S84–S86 and Note S2. b Production of TIIGAs by constitutive and inducible expression of acetyltransferases, and by integration of key enzyme expression cassettes at different loci (H1, H2, EGFP and RFP). Cs+C4, CsSDR and AKR1C4; Con, constitutive; Ind, inducible expression. All data represent the mean of three independent samples, and the error bars indicate the standard deviation.

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