Fig. 1: NETs are enriched in the liver and promote metastases of breast cancer. | Cell Discovery

Fig. 1: NETs are enriched in the liver and promote metastases of breast cancer.

From: Breast cancer induces CD62L+ Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis

Fig. 1

a NET abundance by IF analysis of Ci-H3 and MPO in primary tumor (PT) and LN, lung, bone and liver metastases (met.) of human breast cancer in the Qilu cohort. P values are calculated in comparison to liver metastases. b NET abundance in primary tumors, lung and liver metastases of C57BL/6 mice at week 6 after orthotopic injection of GFP-labeled AT3 cells. c NET abundance in livers of nude mice with intrasplenic injection of MDA-MB-231 (MDA231) and its liver-tropic subline LvM16 on day 16 after inoculation (top), or in primary tumors with LvM16 orthotopic inoculation on day 50 and in lung with intravenous LvM16 inoculation (bottom) on day 30. P values are calculated in comparison to LvM16 liver metastases. d NETs surrounding or within metastatic foci in livers of nude mice on day 40 after intrasplenic injection of LvM16 (zoomed areas shown on right). e–j GSK484 (GSK) treatment (20 mg/kg) of nude mice after LvM16 intrasplenic inoculation for liver metastasis analyses. Shown are IF analysis of NETs in livers (e, f), in vivo bioluminescent imaging (BLI) signals of livers (g, signal normalized to day 0), ex vivo BLI signals of livers (h), representative images of BLI and liver metastases (i), and overall survival of the mice (j). n = 7 (PT/LN), 20 (lung), 17 (bone) or 33 (liver) patients (a), or 3 (b, f, h), 4 (c), or 6 mice (g, j) per group. P values were obtained by repeated measures two-way ANOVA (g), Log rank test (j) and two-tailed unpaired t-test (others). Scale bars, 20 μm. Data are shown as mean ± SEM (g) or mean ± SD (others).

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