Fig. 5: Tumor induces CD62L+ KCs by DMBT1. | Cell Discovery

Fig. 5: Tumor induces CD62L+ KCs by DMBT1.

From: Breast cancer induces CD62L+ Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis

Fig. 5

a Heatmap of secreted protein-encoding genes differentially expressed in MDA-MB-231 vs LvM16. b Overall survival of breast cancer patients stratified by DMBT1 expression in the UNC cohort (GSE3521, GSE26338). c–e DMBT1 expression in human breast cancer of the Qilu cohort. Shown are tumoral DMBT1 mRNA levels (c) and serological DMBT1 protein levels (d) of those with liver-metastatic or non-metastatic primary tumors, and liver metastasis-free survival of patients stratified by tumoral DMBT1 expression (e). f DMBT1 mRNA and protein expression in MDA-MB-231 sublines. g–i CD62L induction (g, h) and Ccl8 expression (i) of KCs treated with CM of the indicated tumor cells with or without DMBT1 overexpression or knockdown. j NETosis of neutrophils cultured with CM from the KCs in i. n = 220 (b), 65 (c, e) and 22 (d) patients, and 3 biological repeats (f–j). P values were obtained by log-rank test (b, e) or two-tailed unpaired t-test (others). Data are shown as mean ± SD.

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