Fig. 7: DMBT1 regulates KCs via MUC1-NF-κB signaling. | Cell Discovery

Fig. 7: DMBT1 regulates KCs via MUC1-NF-κB signaling.

From: Breast cancer induces CD62L+ Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis

Fig. 7

a Co-IP assays for DMBT1 and MUC1-C in 293 T and HL60 cells. Ctrl, Ni-NTA bead control sample. b p65 phosphorylation and nuclear distribution of MUC1-C in KCs after treatment of CM of cancer cells with or without DMBT1 knockdown/overexpression. c ChIP-qPCR analysis of p65 binding to Ccl8 or Cd62l promoters in monocytes. d p65 phosphorylation and CCL8 expression of KCs cultured with DMBT1-overexpressing MCF7 CM and/or JSH-23 (30 μM). e–g CD62L induction (e), Ccl8 and Cd62l expression (f) of KCs after treatment of LvM16 CM, with or without GO203, and the NET-inducing effects of the CM of such KCs (g). h–m GO203 treatment (15 mg/kg) of NOD-SCID mice with intrasplenic injection of MCF7 cells with DMBT1 overexpression. Shown are flow cytometry analysis of CD62L+ KCs in livers (h), in vivo BLI (i), ex vivo BLI of livers (j), representative of BLI and metastases (k), NETosis in livers (l) and body weight changes of mice (m) at week 4 after cancer inoculation. n = 3 biological repeats (c, e–g), 7 (h, i, m), 6 (l) or 3 (j) mice. P values were obtained by two-tailed unpaired t-test (others). Scale bars, 50 μm. Data are shown as mean ± SD (others).

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