Fig. 3: Impact of maternal hyperglycemia on fetal PGCs at E12.5 to E16.5 development stage by RNA-seq. | Cell Discovery

Fig. 3: Impact of maternal hyperglycemia on fetal PGCs at E12.5 to E16.5 development stage by RNA-seq.

From: Intrauterine hyperglycemia impairs mouse primordial germ cell development and fertility by sex-specific epigenetic reprogramming interference

Fig. 3

a Flowchart overview of fetal PGCs isolation from E12.5 to E16.5. b Average number of Oct4-EGFP positive cells in whole gonads at E12.5, E13.5, and E16.5 between the control and IUHG, comprising 10–20 pairs of gonads per group. c PCA analysis of the developmental trajectory of fetal PGCs in control and IUHG samples based on RNA-seq data. d Volcano plot depicting DEGs in IUHG female PGCs at E13.5 compared to the control group (upregulated genes in red, downregulated genes in blue). e Heatmap showing expression of upregulated and downregulated genes in control and IUHG at E12.5, E13.5 female (E13.5 F), and E16.5 F PGCs. f GO analysis of DEGs identifies biological processes affected by IUHG at different development stages. g Boxplots of RNA-seq data and qPCR validation for pluripotency- and meiosis-related DEGs in IUHG E13.5 F PGCs compared to controls. h In vitro culture of E12.5 female PGCs for 24 h. i, j qPCR validation of pluripotency-related (i) and meiosis-related (j) genes in HG-treated female PGCs in vitro compared to controls. All error bars represent the mean ± SD of three biological replicates, each derived from PGCs collected from 6–10 pairs of gonads per group. Significance was calculated using an unpaired two-sided Student’s t-test (b, g, i, j); *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.

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