Fig. 4
RGS1 stabilizes XLG2/GPA1–AGB1 interactions through GAP activity a, b RGS1 is required for the stability of XLG2–AGB1 (a) and GPA1–AGB1 (b) interactions. The indicated constructs were transiently expressed in Col-0 or rgs1-2 protoplasts, and co-IP was performed. c, d The GAP activity of RGS1 is required for the stability of XLG2–AGB1 (c) and GPA1–AGB1 (d) interactions. Co-IP assays were performed as in a. e Disruption of nucleotide-binding motifs in XLG2 stabilizes its interaction with AGB1 independent of RGS1. The indicated constructs were transiently expressed in Col-0 or rgs1-2 protoplasts, and co-IP was performed. Numbers on top of the gel blots indicate arbitrary densitometry units of co-IP products normalized to the amounts of AGB1-HA protein. The experiments were performed twice with similar results
