Fig. 3
From: Tissue clearing of both hard and soft tissue organs with the PEGASOS method
PEGASOS passive immersion procedure achieves whole-body transparency of mouse pups and enables whole-head imaging with no blind area. a, b Mouse pups of P7 age were cleared following the passive immersion procedure. c A Tie2-Cre;Ai14 mouse pup (P7) after clearing was imaged with a 5× objective on a two-photon microscope. SHG signal was displayed in blue. TdTomato signal was displayed in red. Space between the brain and calvarial bone was caused by brain shrinkage. d–k Optical sections at different Z-depths, including the dorsal surface (d), 2.0 mm (e), 3.0 mm (f), 5.0 mm (g), and the ventral surface (h), were acquired. Dotted box in f was enlarged in the insert to show blood vessels. Dotted box in g was enlarged in i to show blood vessels within the tooth germ (arrows). X-Z optical slices acquired at different locations were displayed in j, k. SHG, second harmonic generation signal. Scale bars in c–h, j and k, 5 mm
