Fig. 5
From: Tissue clearing of both hard and soft tissue organs with the PEGASOS method
Modified PEG components within the BB-PEG medium preserves endogenous fluorescence for a long-term. Intestine samples were harvested from CAG-EGFP or Tie2-Cre; Ai14 mice and cleared following indicated clearing methods. a GFP or tdTomato fluorescence intensity changes after each treatment step of PEGASOS. Twelve hours after fixation was recorded as the starting point. Each step lasted for 1 day. b Impact of 4 days of EDTA treatment on GFP or tdTomato fluorescence of fixed intestine samples. c, d GFP or tdTomato fluorescence intensity changes within different clearing media. We record 1 h after the samples being placed into the clearing medium as D0. e Representative images at various time points were acquired with the same exposure conditions. f Intestine samples were placed in complete BB-PEG medium or benzyl benzoate after the PEGASOS clearing for quantitative comparison. GFP fluorescence was preserved in BB-PEG medium, but rapidly quenched by benzyl benzoate. g To evaluate the impact of various forms of PEG on the fluorescence intensity, dehydrated intestine samples were placed in BB-PEG clearing medium of different formulations (benzyl benzoate (75% v/v) + various PEGs (25% v/v)). Fluorescence intensities were measured 1 week after clearing. All values are mean ± s.d. Statistical significance (**P < 0.01; *P < 0.05) was assessed by one-way ANOVA. Scale bars in f, 1 mm
