Fig. 7
From: Tissue clearing of both hard and soft tissue organs with the PEGASOS method
Imaging of the whole-brain vasculatures at sub-cellular resolution. Brains of adult Tie2-Cre;tTAflox;tetO-H2BGFP (TTH) mice were cleared and imaged with a tiling light-sheet microscope. Each dots represent nuclei of an endothelial cell. a A side view of the whole-brain image with dorsal-ventral thickness of ~4 mm. Dorsal view (b), optical sections acquired at 1 mm (c), 2 mm (d), 3 mm (e), and 4 mm (f) were displayed. Boxed region in e was enlarged in e’. g An X-Z optical slice acquired at the hippocampus position was displayed. Boxed areas in g were enlarged in g1 and g2, respectively. Boxed regions in g1 and g2 were enlarged in g1’ and g2’, respectively. h A sagittal Y-Z optical slice acquired at near the midline region
