Fig. 7
From: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP+ cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, 150 μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons (d), immature excitatory neurons (Ex_1/2, e) and mature excitatory neurons (Ex_3/4, f). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH. h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S6c. Scale bar, 50 μm
