Fig. 1
PTEN-L resides at the outer mitochondrial membrane. a Domain structure of PTEN-L protein. b Cell fractionation was performed to isolate mitochondria in HeLa cells. Tim23 and GAPDH were used as mitochondrial and cytosolic markers, respectively. C cytosol, M mitochondria. c Relative ratios of PTEN-L and PTEN in cytosol and mitochondria were calculated with normalization of volume. d Topology assay showing PTEN-L localization at the OMM. Mitochondria were isolated from YFP-Parkin-HeLa cells stably expressing Flag-PTEN-L and treated with different doses of proteinase K and digitonin. e Immunogold EM. YFP-Parkin-HeLa cells stably expressing Flag-PTEN-L were treated without (A, B) or with CCCP (5 µM) (C, D) for 4 h. Cells were subjected to immunoelectron microscopy with anti-Flag antibody. Panels (B) and (D) are the magnified images of boxes in (A) and (C), respectively. Arrows indicate ER structure
