Fig. 3
From: Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis
Characterization of dynamic gene expression patterns in male germ cell development. a Principal component analysis (PCA) of the spermatogenic cells at 20 different stages based on their gene expression pattern exhibited by PC1 and PC2. The variation values of PC1 and PC2 are 39.7 and 5.8%, respectively. Distinct cell types are shown in different colors. b The t-distribute stochastic neighbor embedding (t-SNE) plot with seven clusters of spermatogenic cells (left panel) and their corresponding developmental stages (right panel). Clusters C1 to C7 are shown in different colors. Cells at different developmental stages are shown in different colors as in a. c Line graph showing average gene expression level of down- (left panel) and up-regulated (right panel) differentially expressed genes (DEGs) when comparing each of two consecutive clusters. y axis, log2(TPM/10 + 1). The DEG number of each group is shown in brackets. d Gene ontology (GO) analysis of down-regulated DEGs from clusters C2 to C3 (upper panel), down-regulated DEGs from C3 to C4 (middle panel) and up-regulated DEGs from C4 to C5 (bottom panel), respectively. The dynamic gene expression patterns of these three groups are shown in c. e Hematoxylin and eosin (H & E) staining of wild-type control and Fbxo47-cKO testis sections at 8 weeks old. In mutant testes, seminiferous epithelium was arrested at stage IV. IV: stage IV. Scale bar, 50 μm. f Immunohistochemical staining for the mid-late pachytene spermatocyte marker histone variants H1t (green), γH2AX (red), and DAPI (blue) in sections of 8-week-old wild-type control and Fbxo47-cKO testes. Scale bar, 50 μm
