Fig. 7
From: STAT3 associates with vacuolar H+-ATPase and regulates cytosolic and lysosomal pH
Lysosomal STAT3 protects cells against cytosolic acidification. a Cytosolic pH in the indicated HeLa clones determined by image analysis of cells loaded with pHrodo™ Green AM. Standard curve is displayed on the right. b, c Cell death of the indicated HeLa cell clones (b) and HeLa-STAT3-KO-11 cells transiently transfected with vector or STAT3-Y705F (c) left untreated, starved in EBSS for 6 h, or treated with 25 µM EIPA + 50 mM propionate for 24 h was determined by propidium iodine uptake. Error bars, SD of ≥ 3 independent experiments. P-values were calculated by one-way ANOVA combined with Dunnett’s multiple comparisons test (a, b) or by two-tailed, homoscedastic Student’s t-test (c)
