Fig. 3

Requirement of DH44 signaling for the increase in food consumption by dietary amino acids. a Changes in food consumption by the addition of different concentrations of amino acid mixture to 400 mM sucrose consumed by flies with pan-neuronal RNAi knockdown of the indicated neuropeptide receptors (n = 19–35). Food consumption of 400 mM sucrose alone is used as the baseline (grey, dotted line). b Genomic structure of DH44-R1 and DH44-R2 genes. Dotted boxes outline the deleted DNA fragments by CRISPR/Cas9. c, d Volume of 400 mM sucrose (grey) or 400 mM sucrose plus different concentrations of amino acid mixture (blue) consumed by flies with the indicated genotypes (n = 19–28). e Genomic structure of DH44 genes. Dotted line outlines the indel site induced by CRISPR/Cas9. f Volumes of 400 mM sucrose (grey) or 400 mM sucrose plus different concentrations of amino acid mixture (blue) consumed by flies with the indicated genotypes (n = 19–28). Virgin females were used for all experiments shown in this figure. Data are shown as means ± SEM. NS, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001