Fig. 5

Ex vivo calcium imaging of DH44⁺ neurons. a Schematic diagram of ex vivo calcium imaging. b The ex vivo calcium responses of DH44⁺ neurons by the perfusion of 500 μM L-Glu. Scale bar, 10 μm. c Representative traces of ex vivo calcium responses of DH44⁺ neurons during perfusion of 500 μM L-amino acids. Note that in the ex vivo setup, different DH44⁺ neurons exhibited calcium oscillations or tonic calcium responses upon amino acid stimulation. d–g Quantification of the ex vivo calcium responses of DH44⁺ neurons to different concentrations of the indicated amino acids (n = 9–40). h Quantifications of the ex vivo calcium responses of DH44⁺ neurons to 500 μM of the indicated amino acids in the absence or presence of 1 μM TTX (n = 10–14). i Quantifications of the ex vivo calcium responses of DH44⁺ neurons to L-Glu and D-glucose (n = 10–31). Virgin females were used for all experiments shown in this figure. Data are shown as means ± SEM. NS, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001