Fig. 1
From: Cryo-EM reveals ligand induced allostery underlying InsP3R channel gating
The cryo-EM structure of InsP3R1 channel bound with AdA. a The cryo-EM density map of the InsP3R1-AdA complex is viewed from cytosol along its four-fold axis (left) and along the membrane plane (right). The map is filtered to 4.1 Å and corrected with a temperature factor of −100 Å2. The domains within each protomer are delineated by different colours. Densities corresponding to AdA are coloured magenta. b Zoomed-in view of the AdA density present in the ligand-binding pocket. AdA-InsP3R1 structure is overlaid on the corresponding density map and coloured by domain; the AdA molecule is fit in the density adjoining the LBDs. c AdA molecule is shown in the InsP3R1 binding pocket and is overlaid with densities (mesh) from the difference map (4σ). Residues within 5 Å distance from the docked AdA molecule are labeled and coloured magenta
