Fig. 6
Dynamic features of NDRs at TSSs and TESs in growing mouse oocytes. a The number of total NDRs and NDRs overlapped with alternative promoters at each stage. P-values were defined by the chi square test. b Representative loci showing RefSeq genes, transcripts, alternative TSSs, NDRs and broad peaks of H3K4me3 at each stage. Alternative TSSs are labelled as blue shadows. The starting point and direction of red arrows at RefSeq panel represented the gene transcriptional starting site and gene transcriptional direction. c The average chromatin accessibility of RefSeq genes across gene bodies at each stage. d Representative loci showing gene TESs with NDRs. TESs are labelled with orange shadows. The starting point and direction of red arrows at RefSeq panel represent the gene transcriptional starting site and gene transcriptional direction. e Alluvial map showing the dynamics of genes with both TSS- and TES-NDRs, genes with only TSS-NDRs, genes with only TES-NDRs and genes with no NDR. TSS-NDRs and TES-NDRs were defined as NDRs overlapped with TSS regions (200 bp upstream and 100 bp downstream of the TSS) and TES regions (200 bp upstream and 100 bp downstream of the TES). Only genes with both covered TSS and TES regions (containing at least five GCH sites) were used. Bars denote the number of genes in each category at growing stage, and lines between bars denote the number of genes transition from a certain category at earlier stage to another category at later stage. f Expression levels of genes with both TSS and TES NDRs, genes with only TSS-NDRs, genes with only TES-NDRs and genes with no NDR at the NGO and FGO stage. P-values were defined by the two-tailed Student’s t-test
