Fig. 4

Baf60c is a key Isl1 downstream target. a Scheme depicting distinct stages of directed cardiac differentiation (top). Relative mRNA expression of selected cardiomyocyte contraction and structural genes at day 10 of cardiac differentiation after Isl1 KD at day 4 (left panel) or at day 8 (right panel). Data are presented as mean ± SEM, n = 3. b Heatmap representation of RNA-Seq analysis of FACS-sorted Nkx2-5⁺ CPCs derived from wild-type and Isl1^−/− ESCs (n = 2; fold change >1.5; log2 fold change <-0.58, >0.58; p-value < 0.05). c Overlap between genes bound by Isl1 in mESC-derived CPCs and differentially expressed in sorted Isl1^−/− compared to control CPCs. d Examples of genes regulated and bound by Isl1 in mESC-derived CPCs, showing genome tracks of Isl1 ChIP-Seq and RNA-Seq reads of sorted control and Isl1^−/− mESC-derived CPCs. e GO terms enriched in genes bound by Isl1 in mESC-derived CPCs and downregulated in Isl1 knockout CPCs. f WT and Isl1 knockout E9.5 embryos viewed from the left (left panels) and the right (right panels) after in situ hybridization with a Baf60c probe. Scale bars, 200 µm. Abbreviations: rv, right ventricle; lv, left ventricle; OFT, outflow tract. g Heatmap representation of RNA-Seq analysis of FACS-sorted Nkx2-5⁺ CPCs derived from control and Baf60c knockdown mESCs (n = 2; fold change >1.5; log2 fold change <−0.58, >0.58; p-value < 0.05). h Overlap of deregulated genes in sorted Isl1^−/− and Baf60c knockdown CPCs. i Heatmap of genes downregulated or upregulated in both Isl1 knockout and Baf60c knockdown versus control CPCs. Representative genes and enriched GO terms are presented on the right side