Fig. 1
From: AIF-regulated oxidative phosphorylation supports lung cancer development
Reduced lung cancer in Aif-deficient KrasG12D mice. a Aif deletion significantly prolongs the survival of mice infected with Ad5-CMV-Cre in comparison to their Aif WT controls. Kaplan–Meier plot. P = 0.0022 (log rank test) for Aiffl/y KrasG12D (n = 12) versus Aif+/y KrasG12D (n = 15) littermates. b Representative lung tumor sections (H&E staining) in Aiffl/y KrasG12D and Aif+/y KrasG12D littermates at the indicated time points after Ad5-CMV-Cre inhalation. Scale bar, 2 mm. c Quantification of overall tumor burden. Total tumor areas comprising hyperplasia, adenomas, and adenocarcinomas, were scored automatically by a Definiens software algorithm. Three planes from each lung were stained with H&E and analyzed in a blinded fashion. Data are shown as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test). n = 5 per genotype for each time point. d Micro-CT analysis of lung tumors of Aiffl/y KrasG12D and Aif+/y KrasG12D littermate mice, analyzed at the indicated weeks after Ad5-CMV-Cre inhalation. Representative data from individual mice are shown. Scale bar, 2 mm
