Fig. 3
From: AIF-regulated oxidative phosphorylation supports lung cancer development
AIF deficiency enhances glycolysis and sensitivity to glucose deprivation. a, b ECAR in pneumocytes isolated from Aif+/y KrasG12D and Aiffl/y KrasG12D mice 6 weeks after Ad5-CMV-Cre inhalation. Basal glycolytic rate after stimulation with glucose (a) and change in ECAR over baseline after oligomycin treatment (b). *P < 0.05; ***P < 0.001 (Unpaired two-sided t-test). c, d pH measurements (c) and lactate production (d) in the culture media of primary pneumocytes isolated from Aif+/y KrasG12D and Aiffl/y KrasG12D mice 6 weeks after Ad5-CMV-Cre inhalation. Cells were seeded on day 0 with a density of 2.5 × 105 cells/well in a 6-well plate. Data are shown as means ± SEM. n = 5 per genotype. **P < 0.01; ***P < 0.001 (Two-way ANOVA test). e Aif+/y KrasG12D and Aiffl/y KrasG12D pneumocytes were cultured for 72 h in the presence of the indicated concentrations of 2-DG followed by staining with PI to determine the frequency of dead cells. ***P < 0.001 (two-way ANOVA, Bonferroni’s post hoc test). f Aif+/y KrasG12D and Aiffl/y KrasG12D pneumocyte growth in the absence of glucose. Cells were cultured for three days in the presence (5 g/L) or absence of glucose and their viability was determined. g Reduced ATP production in Aiffl/y KrasG12D cells upon glucose withdrawal. Pneumocytes were cultured for 36 h in the absence or presence of glucose and intracellular ATP levels were determined among the viable cell fractions. ATP content was normalized to the protein concentration of the samples. Data are shown as means±SEM. n = 5 per genotype. *P < 0.05; **P < 0.01; ***P < 0.001 (two-way ANOVA, Bonferroni’s post hoc test)
