Fig. 5

Stable Aif depletion impairs colony formation of both KRAS WT and KRAS-mutated human lung cancer cells. KRAS WT cells (H1437 and H1975) and KRAS-mutated human lung cancer cell (H460, H727, A427, H1650, and H358) were transduced with lentiviral vectors expressing shAIF-GFP (or shSCR-GFP as a control), and GFP-positive clones were selected by cytofluorometric sorting. A549 cells were used as an internal control. a Representative images of different 96-well plates seeded with one GFP-positive cell per well generated from different cell lines. The images were acquired after 7–12 day-long cell culture. b–d Quantification of average GFP⁺ clone area (b), total GFP⁺ clone area (c), average fluorescence intensity (d). The values obtained for the control shSCR were set as 100, and the values of shAIF were normalized to individual shSCR controls. Results are expressed as means ± SEM of a representative experiment (experiments were done in triplicates). *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)