Fig. 4
Comparable CYP450 drug-metabolizing activities of hiHeps to PHHs. a RT-qPCR analysis of major CYP450s and UGT1A1 in HEFs (n = 3), HepG2 cells (n = 2), hHPLCs (n = 2), hiHeps derived from hHPLCs at different passages (n = 2), F-PHHs (n = 5) and ALs (n = 4). Relative expression was normalized to F-PHHs. b Immunofluorescence staining of key CYP450 enzymes (CYP3A4, CYP2C9, CYP2C19, CYP2C8, CYP2D6, CYP1A2) and UGT1A1 in hiHeps derived from hHPLCs at P5. c UPLC/MS/MS analysis in hiHeps derived from hHPLCs at different passages, HepG2 cells and F-PHHs for the drug-metabolic activities of 7 CYP450s. n = 3. d UPLC/MS/MS analysis in hiHeps, HEFs cells and F-PHHs for the drug-metabolic activities of UGTs. n = 3. Data not detected were labeled as “n.d.”. For all measurements, ‘n’ represents the number of biological replicates. The scale bars represent 50 μm. Data are presented as mean ± SEM
