Fig. 1
From: Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing
Transcriptomic identification of different cell populations in human CS 13 dorsal aorta (DA) and the capture of HECs. a Identification of cell populations in CS 13 DA visualized by UMAP. Each dot represents one cell and colors represent cell clusters as indicated. b UMAP visualization of the expression of curated feature genes for the identification of cell clusters (CDH5, SPI1, PDGFRA and EPCAM). c Violin plots showing the expression of feature genes in each cell cluster. Colors represent cell clusters indicated in a. d Pie chart showing the percentages and absolute numbers of each cell cluster involved in CS 13 DA. e UMAP visualization of AEC, HEC and HC clusters, resulted from sub-dividing the cells in AEC and Hem clusters described in a as indicated in the lower right frame. f Heatmap showing the scaled expression of top 10 differentially expressed genes (DEGs) in AEC, HEC and HC clusters. g The major Gene Ontology biological process (GO:BP) terms in which DEGs are enriched for each cluster. h Dot plots showing the scaled expression level of top 10 significantly differentially expressed surface marker genes in AEC, HEC and HC. Note no specific marker for HEC and only nine genes for HC cluster met the criteria. Colors represent the scaled expression and size encodes the proportion of gene-expressing cells
