Fig. 5
From: Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing
Computational analysis of the heterologous cellular interactions for the development of HSC-primed HECs. a The schematic diagram indicates the heterologous cellular interactions of ligand–receptor pairs between HEC and other clusters including EC, AEC, epithelial and four mesenchymal cell clusters. The direction of arrows indicates from the ligands to the corresponding receptors. Different colors of arrows indicate different databases for ligand–receptor analysis. The thickness of arrows indicates the relative number of ligand–receptor pairs detected. b Frequency of ligand–receptor genes involved in KEGG pathways. c Heatmap showing the average expression of molecules for each ligand–receptor pair in distinct stromal clusters when coupled with HEC. The hierarchical clustering result of cell populations (columns) and ligand–receptor pairs (rows) is shown. d The expression of ligand (blue) and its receptor (red) genes of indicated pairs. Dashed line frames indicate the populations in which the expression of the given gene met the criteria. e Dot plots showing the ligand–receptor pairs between EC/AEC as ligand-expressing cell and HEC as receptor-expressing cell
