Fig. 2
From: Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana
More cytokinin response is observed at the lower water potential side of the root after hydrostimulation treatment. a–d Cytokinin response was visualized by a confocal microscope using TCSn::GFP transgenic plants. Roots were hydrostimulated for 60 min before the images were taken. In control medium (1/2 MS split-agar medium), levels of GFP signal are similar at both sides of the root tips (a, c). Lower water potential side showed more GFP signal in the hydrostimulated Col-0 but not miz1-2 roots (b, d). e–h GFP signals (a–d) were merged with their corresponding bright-field pictures. i GFP fluorescence ratio of right side versus left side of lateral root caps (controls), or between convex side and concave side of lateral root caps (hydrostimulated seedlings) within a 200-μm meristematic zone starting from the quiescent center. Each circle represents the data from an individual root. Boxplots span the first to the third quartiles of the data. Whiskers indicate minimum and maximum values. A line in the box represents the mean. “n” represents the number of roots used in this experiment. Scale bars represent 50 µm. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses. P < 0.001
