Fig. 3
From: ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer
ILF3 directly regulates SGOC gene mRNA stability. a ILF3 binds to the mRNAs of SGOC pathway genes. RNA immunoprecipitation (RIP) was performed in DLD1 cells using anti-ILF3, anti-SNRNP70 (control) or anti-IgG antibodies, followed by RT-qPCR with primers recognizing the indicated mRNAs. The fold expression of RIP-enriched mRNAs relative to the input was calculated. The data are presented as the means ± SD. b RT-PCR of serine pathway genes in DLD1 and HCT-8 cells transduced with ILF3 shRNA followed by actinomycin D treatment (10 μg/mL) over time. The data are presented as the means ± SD. c A schematic drawing of ILF3 RBM-truncated mutants and their expression. Asterisk indicates non-specific band. d The decreased production of SAM in ILF3 KD cells could be rescued by reintroduction of WT ILF3 but not the ILF3 dsRBM mutants. The data are presented as the means ± SD. **P < 0.01. e The decreased gene expression levels of PHGDH, PSAT1, PSPH, SHMT1 and SHMT2 in ILF3-KD cells could be rescued by reintroduction of ILF3 WT but not RBM-truncated mutants. The data are presented as the means ± SD. *P < 0.05; **P < 0.01. f Correlation analyses of ILF3 and SGOC genes in 521 CRC patients. The human CRC patient dataset was obtained from TCGA.
