Fig. 2: Key features of the LR in the cryo-ET reconstruction are confirmed by an independent cryo-EM reconstruction of the X. laevis NPC.
From: Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex
a Reconstruction of a representative NPC particle by sub-tomogram averaging (STA). A top view and a tilt-45° view are shown. The individual subunits of the CR, IR, NR and LR were projected back into the original tomograms to allow reconstruction of a number of NPC particles. Shown here is a representative NPC particle, which contains four ring scaffolds: CR (colored yellow), IR (pink), NR (cyan) and LR (marine). Viewed perpendicular to the NE (left panel), the IR and LR define the inner and outer diameters, respectively, of the cylindrical NPC. b Reconstruction of the NPC by single particle analysis (SPA). CR, IR and NR were reconstructed using the C8 symmetry. The LR was reconstituted using the refined LR subunit and the C8 symmetry. The LR is highlighted. c The key structural features of the LR subunit are nearly identical between the STA reconstruction (marine, top panel, accession code EMD-0983) and the SPA reconstruction (gray, bottom panel, accession code EMD-0982). Their overlay is shown in the middle panel. Scale bar, 10 nm.
