Fig. 6: MiDAS regions are mostly devoid of DNA replication origins. | Cell Research

Fig. 6: MiDAS regions are mostly devoid of DNA replication origins.

From: High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing

Fig. 6

a Top panels, MiDAS signal (purple) and nascent transcription (green, forward transcription; red, reverse transcription) of non-transfected U2OS cells. Bottom panels, origin firing profiles (olive-green) of control (Ctrl) and RIF1-depleted U2OS cells at the EXOC4 locus. For origin firing, the cells were synchronized by mitotic shake-off, released from the mitotic block and allowed to progress through G1 and into S phase over 14 h in the presence of EdU, to label nascent DNA, and hydroxyurea, to limit fork progression. Under these conditions, in control cells, origin firing is observed only in the early S-replicating part of the genome. Bin resolution and ruler scale are as in Fig. 1b. Tx, transcription. b Genome-wide average origin firing signal (green) in control and RIF1-depleted cells at double-peak (left panels) and merged- and single-peak (right panels) MiDAS regions, in comparison to the genome-wide average MiDAS signal (purple). Span of genomic region, 2.9 Mb (double-peak) or 2.3 Mb (merged- and single-peak); Pk, peak.

Back to article page